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KMID : 0877720090130020142
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Journal of Korean Continence Society
2009 Volume.13 No. 2 p.142 ~ p.151
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The Beneficial Effect of Korean Red Ginseng on Rat Bladder Contractility and Oxidant Damage Following Ischemia/Reperfusion
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Shin Ju-Hyun
Oh Tae-Hoon
Yang Seung-Woo
Youn Chang-Sik
Kim Young-Ho
Hwang Eu-Gene
Kim Yong-Woong
Yuk Seung-Mo
Lim Jae-Sung
Song Ki-Hak
Sul Chong-Koo
Na Yong-Gil
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Abstract
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Purpose: N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder.
Materials and Methods: I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups: sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation.
Results: In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group.
Conclusion: Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R.
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KEYWORD
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Bladder, Ischemia, Reperfusion, N-acetylcysteine
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